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Filgen Inc mircury lna array mirna profiling service
Mircury Lna Array Mirna Profiling Service, supplied by Filgen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Article Title: Diet-Induced Obesity Modulates Epigenetic Responses to Ionizing Radiation in Mice
Article Snippet: Total RNA was extracted from the livers of C57BL/6J mice ( n = 3 per experimental condition) using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. The profiling of miRNA expression levels was performed using the miRCURY LNA miRNA profiling service (Filgen). .. The quality of total RNA was checked using a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA), then RNA was labeled with Hy3 and hybridized to miRCURY LNA microRNA arrays 7 th generation (Exiqon, Vedbaek, Denmark).



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 miRNA  regulations in the livers of non-irradiated and irradiated C57BL/6J mice fed a normal or HF diet, determined using Significance Analysis of Microarrays (SAM).

Journal: PLoS ONE

Article Title: Diet-Induced Obesity Modulates Epigenetic Responses to Ionizing Radiation in Mice

doi: 10.1371/journal.pone.0106277

Figure Lengend Snippet: miRNA regulations in the livers of non-irradiated and irradiated C57BL/6J mice fed a normal or HF diet, determined using Significance Analysis of Microarrays (SAM).

Article Snippet: The profiling of miRNA expression levels was performed using the miRCURY LNA miRNA profiling service (Filgen).

Techniques: Irradiation

Up-regulated  miRNA  profiles of porcine pituitary cell after GnRH treatment.

Journal: PLoS ONE

Article Title: Differentially Expressed miRNAs after GnRH Treatment and Their Potential Roles in FSH Regulation in Porcine Anterior Pituitary Cell

doi: 10.1371/journal.pone.0057156

Figure Lengend Snippet: Up-regulated miRNA profiles of porcine pituitary cell after GnRH treatment.

Article Snippet: The miRNA expression was profiled using Exiqon's miRCURY LNA Array miRNA profiling services (Exiqon, Denmark).In brief, small RNAs were enriched by miRNeasy mini kit (QIAGEN),RNA quality and quantity were measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.

Techniques:

Down-regulated  miRNA  profiles of porcine pituitary cell after GnRH treatment.

Journal: PLoS ONE

Article Title: Differentially Expressed miRNAs after GnRH Treatment and Their Potential Roles in FSH Regulation in Porcine Anterior Pituitary Cell

doi: 10.1371/journal.pone.0057156

Figure Lengend Snippet: Down-regulated miRNA profiles of porcine pituitary cell after GnRH treatment.

Article Snippet: The miRNA expression was profiled using Exiqon's miRCURY LNA Array miRNA profiling services (Exiqon, Denmark).In brief, small RNAs were enriched by miRNeasy mini kit (QIAGEN),RNA quality and quantity were measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.

Techniques:

Red spots represent up-regulated miRNAs, while blue spots indicate down-regulated miRNAs. The spots in picture were plotted according to their relative position in chromosome length.

Journal: PLoS ONE

Article Title: Differentially Expressed miRNAs after GnRH Treatment and Their Potential Roles in FSH Regulation in Porcine Anterior Pituitary Cell

doi: 10.1371/journal.pone.0057156

Figure Lengend Snippet: Red spots represent up-regulated miRNAs, while blue spots indicate down-regulated miRNAs. The spots in picture were plotted according to their relative position in chromosome length.

Article Snippet: The miRNA expression was profiled using Exiqon's miRCURY LNA Array miRNA profiling services (Exiqon, Denmark).In brief, small RNAs were enriched by miRNeasy mini kit (QIAGEN),RNA quality and quantity were measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.

Techniques:

This figure was drawn according to KEGG pathway (ssc4912), red arrow near the box (general on the right) represents up-regulated miRNAs while blue arrow represents down-regulated miRNAs. The number near the arrow indicates number of miRNAs targeting this gene or its family. For example, 10 up-regulated miRNAs and 3 down-regulated miRNAs were predicted to target FSHβ mRNA 3′-UTR.TF indicates transcription factor involved in FSHβ transcription.

Journal: PLoS ONE

Article Title: Differentially Expressed miRNAs after GnRH Treatment and Their Potential Roles in FSH Regulation in Porcine Anterior Pituitary Cell

doi: 10.1371/journal.pone.0057156

Figure Lengend Snippet: This figure was drawn according to KEGG pathway (ssc4912), red arrow near the box (general on the right) represents up-regulated miRNAs while blue arrow represents down-regulated miRNAs. The number near the arrow indicates number of miRNAs targeting this gene or its family. For example, 10 up-regulated miRNAs and 3 down-regulated miRNAs were predicted to target FSHβ mRNA 3′-UTR.TF indicates transcription factor involved in FSHβ transcription.

Article Snippet: The miRNA expression was profiled using Exiqon's miRCURY LNA Array miRNA profiling services (Exiqon, Denmark).In brief, small RNAs were enriched by miRNeasy mini kit (QIAGEN),RNA quality and quantity were measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.

Techniques:

Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. Arrows represent transcription factor-gene regulation, while dark lines represent miRNA regulation on mRNA except c-Jun and c-Fos.

Journal: PLoS ONE

Article Title: Differentially Expressed miRNAs after GnRH Treatment and Their Potential Roles in FSH Regulation in Porcine Anterior Pituitary Cell

doi: 10.1371/journal.pone.0057156

Figure Lengend Snippet: Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. Arrows represent transcription factor-gene regulation, while dark lines represent miRNA regulation on mRNA except c-Jun and c-Fos.

Article Snippet: The miRNA expression was profiled using Exiqon's miRCURY LNA Array miRNA profiling services (Exiqon, Denmark).In brief, small RNAs were enriched by miRNeasy mini kit (QIAGEN),RNA quality and quantity were measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.

Techniques: